RNA N - glycosidase and anti - tumor activities
نویسندگان
چکیده
1. Abstract 2. Introduction 3. Material and methods 3.1. Cloning and sequencing of TYchi gene 3.2. Southern and northern blot hybridization 3.3. Expression and Purification of Recombinant TYchi 3.4. Chitinase enzymic assay of TYchi 3.5. Assay for RNA N-glycosidase activity 3.6. Assay for cell-free translation-inhibiting activity 3.7. Assay for cytotoxic activity on human tumor cells 3.8. Modeling of the three-dimensional structure of TYchi 4. Results 4.1. Cloning and characterization of TYchi genes 4.2. Protein expression and chitinase activity analysis 4.3. RNA N-glycosidase, cell-free translation-inhibiting, and cytotoxic activity analysis 4.4. TYchi 3-D structure analysis 5. Discussion 6. Acknowledgement 7. References
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